Laboratory methods in enzymology : DNA /
edited by Jon Lorsch.
- First edition.
- 1 online resource (lvi, 348 pages) : illustrations.
- Methods in enzymology, volume 529 0076-6879 ; .
- Methods in enzymology ; v. 529. .
Includes bibliographical references and indexes.
PCR primer design / How plasmid preparation kits work / Introducing exogenous DNA into cells / Agarose gel electrophoresis / Analysis of DNA by southern blotting / Purification of DNA oligos by denaturing polyacrylamide gel electrophoresis (PAGE) / Molecular cloning / Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway® recombination cloning technology and Flp-In T-REx® lines / Restrictionless cloning / Isolation of plasmid DNA from bacteria / Preparation of genomic DNA from bacteria / Isolation of genomic DNA from mammalian cells / Sanger dideoxy sequencing of DNA / Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform / Next generation sequencing / Generating mammalian stable cell lines by electroporation / Transient mammalian cell transfection with polyethylenimine (PEI) / Site-directed mutagenesis / PCR-based random mutagenesis / Megaprimer method for mutagenesis of DNA / Troubleshooting PCR / Quantitative PCR / Jessica S. Dymond -- General PCR / Colony PCR / Chemical transformation of yeast / Transformation of E. coli via electroporation / Transformation of chemically competent E. coli / Rubén Álvarez-Fernández -- Laura Koontz -- Laura Koontz -- Laura Koontz -- Gary Glenn and Lefkothea-Vasiliki Andreou -- Sara Lopez-Gomollon and Francisco Esteban Nicolas -- Juliane C. Lessard -- Jessica Spitzer, Markus Landthaler, and Thomas Tuschl -- Mikkel A. Algire -- Lefkothea-Vasiliki Andreou -- Lefkothea-Vasiliki Andreou -- Preparation of genomic DNA from Saccharomyces cerevisiae / Jessica S. Dymond -- Cheryl M. Koh -- Sarah E. Walker and Jon Lorsch -- Srinivasan Yegnasubramanian -- Srinivasan Yegnasubramanian -- Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- Julia Bachman -- Jessica S. Dymond -- Craig W. Vander Kooi -- Kirstie Canene-Adams -- Kirstie Canene-Adams -- Megan Bergkessel and Christine Guthrie -- Megan Bergkessel and Christine Guthrie -- Juliane C. Lessard -- Rachel Green and Elizabeth J. Rogers. Explanatory chapter: Explanatory chapter: Explanatory chapter: Explanatory chapter: Explanatory chapter: Explanatory chapter:
9780124199545 (electronic bk.) 0124199542 (electronic bk.)
Enzymology.
DNA.
Biotechnology--Methodology.
Molecular biology--Methodology.
Sequence Analysis, DNA--methods.
Biotechnology--methods.
Biotechnology--istrumentation.
Molecular biology--methods.
Molecular biology--istrumentation.
SCIENCE / Life Sciences / Biochemistry
Electronic books.
QP601 / .M48 2013eb
572/.7
Includes bibliographical references and indexes.
PCR primer design / How plasmid preparation kits work / Introducing exogenous DNA into cells / Agarose gel electrophoresis / Analysis of DNA by southern blotting / Purification of DNA oligos by denaturing polyacrylamide gel electrophoresis (PAGE) / Molecular cloning / Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway® recombination cloning technology and Flp-In T-REx® lines / Restrictionless cloning / Isolation of plasmid DNA from bacteria / Preparation of genomic DNA from bacteria / Isolation of genomic DNA from mammalian cells / Sanger dideoxy sequencing of DNA / Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform / Next generation sequencing / Generating mammalian stable cell lines by electroporation / Transient mammalian cell transfection with polyethylenimine (PEI) / Site-directed mutagenesis / PCR-based random mutagenesis / Megaprimer method for mutagenesis of DNA / Troubleshooting PCR / Quantitative PCR / Jessica S. Dymond -- General PCR / Colony PCR / Chemical transformation of yeast / Transformation of E. coli via electroporation / Transformation of chemically competent E. coli / Rubén Álvarez-Fernández -- Laura Koontz -- Laura Koontz -- Laura Koontz -- Gary Glenn and Lefkothea-Vasiliki Andreou -- Sara Lopez-Gomollon and Francisco Esteban Nicolas -- Juliane C. Lessard -- Jessica Spitzer, Markus Landthaler, and Thomas Tuschl -- Mikkel A. Algire -- Lefkothea-Vasiliki Andreou -- Lefkothea-Vasiliki Andreou -- Preparation of genomic DNA from Saccharomyces cerevisiae / Jessica S. Dymond -- Cheryl M. Koh -- Sarah E. Walker and Jon Lorsch -- Srinivasan Yegnasubramanian -- Srinivasan Yegnasubramanian -- Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- Julia Bachman -- Jessica S. Dymond -- Craig W. Vander Kooi -- Kirstie Canene-Adams -- Kirstie Canene-Adams -- Megan Bergkessel and Christine Guthrie -- Megan Bergkessel and Christine Guthrie -- Juliane C. Lessard -- Rachel Green and Elizabeth J. Rogers. Explanatory chapter: Explanatory chapter: Explanatory chapter: Explanatory chapter: Explanatory chapter: Explanatory chapter:
9780124199545 (electronic bk.) 0124199542 (electronic bk.)
Enzymology.
DNA.
Biotechnology--Methodology.
Molecular biology--Methodology.
Sequence Analysis, DNA--methods.
Biotechnology--methods.
Biotechnology--istrumentation.
Molecular biology--methods.
Molecular biology--istrumentation.
SCIENCE / Life Sciences / Biochemistry
Electronic books.
QP601 / .M48 2013eb
572/.7