Bibliothèque de L'IMIST/CNRST

Introduction -- Basic molecular biology -- The flow of genetic characters and information -- The structure of nucleic acids -- Organization of the gene -- Expression of the gene -- Gene silencing -- Handling the nucleic acids -- Isolation and purification of DNA and RNA -- Quantification and handling of DNA and RNA -- Radio labeling of DNA and RNA -- Hybidization of nucleic acid -- Gel Electrophoresis -- Sequencing DNA -- Tools used in recombinant DNA technology -- Restriction enzymes -- DNA modifying enzymes -- Recombinant DNA technology: biological aspects -- Host cell types -- Plasmid vectors for use in E. coli -- Bacteriophage vectors for use in E. coli -- Hybrids plasmid/phage vector -- Getting DNA into cells -- Strategies for cloning -- What is the best approach? -- Cloning genes from mRNA -- Use of genomic DNA in cloning -- Advance cloning strategies -- Polymerase chain reaction -- Recombinant analysis: selection and screening -- Selection and screening methods -- Screening using nucleic acid hybridization -- Screening for expressed genes by Immunological assay -- Gene anaysis after cloning -- Genetic engineering in action -- Gene characterization Studying gene expression -- Making proteins from cloned genes -- Expression system based on Baculovirus -- Protein engineering -- Developement of transgenic organisms -- Application of recombinant DNA technology -- Genome and genome analysis -- Potential hazards of recombinant DNA technology -- Appendix: Some protocols used in recombinant DNA technology.