Bibliothèque de L'IMIST/CNRST

Description based upon print version of record.

Front Cover; Metabolomics Coming of Age with its Technological Diversity; Copyright; Contents; Contributors; Chapter One: High-Resolution 1H-NMR Spectroscopy and Beyond to Explore Plant Metabolome; 1. Introduction; 2. NMR Signals: Measurable Properties; 2.1. What is nuclear magnetic resonance spectroscopy?; 2.2. Choosing the isotope of biological interest detected by NMR; 2.3. Choosing the field and probe technology; 2.4. Adapting the extraction method to the biological objective; 2.4.1. Is the extraction step necessary?; 2.4.2. Extraction challenges for high-resolution 1H-NMR spectroscopy

2.4.3. Solvent choice: A critical step for extraction2.4.4. Other determining factors for high-quality 1H-NMR spectrum; 2.5. Choosing the appropriate method for solvent signal suppression; 2.5.1. Using selective pulse sequences; 2.5.2. Using D2O exchange; 2.5.3. Relaxation and diffusion-editing techniques; 2.6. Quantitative 1H-NMR: Precautions during extraction, acquisition and processing; 2.7. Automation of 1H-NMR analysis for high-throughput metabolomics; 2.7.1. Automatic sample preparation and transfer to the spectrometer; 2.7.2. Automatic probe tuning and magnetic field adjustment

2.7.3. Automatic data acquisition and processing3. Metabolomics NMR Fingerprinting for Discriminating Plant Metabolomes; 3.1. Challenging tasks of metabolomics fingerprinting; 3.2. The basis of metabolic fingerprinting; 3.2.1. Design of fingerprinting experiments; 3.2.2. Acquisition and post-treatment of NMR spectra; 3.2.3. Highlighting biomarkers from NMR data with chemometrics; 3.3. A wide range of applications as evidence of success; 3.3.1. Food origin and authentication; 3.3.2. Medicinal plant characterization; 3.3.3. Ecotoxicological and environment effect

3.3.4. Functional genomics and genetics4. Metabolomics NMR Profiling and Spectra Annotation; 4.1. 1D spectra annotation by using spectra libraries of pure compounds; 4.2. Spectra annotation using different multidimensional NMR pulse sequences; 4.3. Resources and data standards for NMR metabolomics; 5. Application of 1H-NMR Profiling; 5.1. A wide range of applications as evidence of success; 5.1.1. Application of 1H-NMR profiling in functional genomics and plant breeding; 5.1.2. Following metabolic adaption in plant submitted to stress conditions

5.1.3. Application of 1H-NMR profiling in production of grapevines and wines5.1.4. Application of 1H-NMR profiling for characterization of medicinal plants; 5.1.5. Application of 1H-NMR in food authenticity and quality control; 6. New Challenges for 1H-NMR Metabolomics; 6.1. Technological challenges; 6.1.1. Cryogenically cooled probes and microprobes; 6.1.2. HRMAS-NMR for intact plant sample studies; 6.1.3. Hyphenated NMR techniques for identification; 6.1.4. Spectra annotation using fast 2D NMR pulse sequences; 6.2. Integrating 1H-NMR with other metabolomics and omics technologies

7. Concluding Remarks

Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 67th volume, the series features several reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This thematic volume features reviews on metabolomics coming of age with its technological diversity.Publishes in-depth and up-to-date reviews on a wide range of topics in plant sciencesFeatures a wide range of reviews by recognized experts on all aspects of pl.

Includes indexes.