Bibliothèque de L'IMIST/CNRST

Includes bibliographical references and indexes.

1. Biochemical methods to monitor autophagy-related processes in yeast / Heesun Cheong, Daniel J. Klionsky -- 2. Viability assays to monitor yeast autophagy / Takeshi Noda -- 3. Quantitative pho8 60 assay of nonspecific autophagy / Takeshi Noda, Daniel J. Klionsky -- 4. Fluorescence microscopy-based assays for monitoring yeast ATG protein trafficking / Takahiro Shintani, Fulvio Reggiori -- 5. Measuring macroautophagy in S. cerevisiae : autophagic body accumulation and total protein turnover / Tanja Prick, Michael Thumm -- 6. Aminopeptidase I enzymatic activity / Peter Schu -- 7. Monitoring autophagy in yeast using FM 4-64 fluorescence / Dikla Journo, Gal Winter, Hagai Abeliovich -- 8. Monitoring mitophagy in yeast / Nadine Camougrand ... [and others] -- 9. Monitoring organelle turnover in yeast using fluorescent protein tags / Rodney J. Devenish ... [and others] -- 10. Electron microscopy in yeast / Misuzu Baba -- 11. Cell-free reconstitution of microautophagy in yeast / Andreas Mayer.

12. Autophagy in wine making / Eduardo Cebollero, M. Teresa Rejas, Ramon González -- 13. Purification and in vitro analysis of yeast vacuoles / Margarita Cabrera, Christian Ungermann -- 14. Pexophagy in Hansenula polymorpha / Tim van Zutphen, Ida J. van der Klei, Jan A.K.W. Kiel -- 15. Pexophagy in Pichia pastoris / Masahide Oku, Yasuyoshi Sakai -- 16. Methods of plate pexophagy monitoring and positive selection for ATG gene cloning in yeasts / Oleh V. Stasyk, Taras Y. Nazarko, Andriy A. Sibirny -- 17. Autophagy in the filamentous fungus aspergillus fumigatus / Daryl L. Richie, David S. Askew -- 18. Monitoring autophagy in the filamentous fungus podospora anserina / Bérangère Pinan-Lucarré, Corinne Clavé -- 19. Monitoring autophagy in magnaporthe oryzae / Xiao-Hong Liu, Tong-Bao Liu, Fu-Cheng Lin -- 20. Methods for functional analysis of macroautophagy in filamentous fungi / Yi Zhen Deng, Marilou Ramos-Pamplona, Naweed I. Naqvi -- 21. Autophagy in candida albicans / Glen E. Palmer -- 22. Analysis of autophagy during infections of cryptococcus neoformans / Guowu Hu, Jack Gibbons, Peter R. Williamson -- 23. Autophagy and autophagic cell death in dictyostelium / Emilie Tresse ... [and others] -- 24. Analysis of autophagy in the enteric protozoan parasite entamoeba -- 25. Kinetoplastida / Viola Denninger ... [and others] -- 26. Methods to investigate autophagy during starvation and regeneration in hydra / Wanda Buzgariu, Simona Chera, Brigitte Galliot -- 27 Autophagy in freshwater planarians / Cristina Gonzalez-Estevéz.

28. Qualitative and quantitative characterization of autophagy in caenorhabditis elegans by electron microscopy / Timea Sigmond ... [and others] -- 29. Monitoring the role of autophagy in C. elegans aging / Alicia Meléndez, David H. Hall, Malene Hansen -- 30. Autophagy in caenorhabditis elegans / Tímea Sigmond ... [and others] -- 31. Chimeric fluorescent fusion proteins to monitor autophagy in plants / Ken Matsuoka -- 32. Use of protease inhibitors for detecting autophagy in plants / Yuji Moriyasu, Yuko Inoue -- 33. Lysosomes and autophagy in aquatic animals / Michael N. Moore ... [and others] -- 34. Autophagy in ticks / Rika Umemiya-Shirafuji, Tomohide Matsuo, Kozo Fujisaki -- 35. Quantitative analysis of autophagic activity in drosophila neural tissues by measuring the turnover rates of pathway substrates / Robert C. Cumming, Anne Simonsen, Kim D. Finley -- 36. Genetic manipulation and monitoring of autophagy in drosophila / Thomas P. Neufeld -- 37. Monitoring autophagy in insect eggs / Ioannis P. Nezis, Issidora Papassideri -- 38. In vitro methods to monitor autophagy in lepidoptera / Gianluca Tettamanti, Davide Malagoli.

Description based on print version record.

This is the companion volume to Daniel Klionsky's Autophagy: Lower Eukaryotes, which features the basic methods in autophagy covering yeasts and alternative fungi (aspergillus, podospora, magnaporthe). Klionsky is one of the leading authorities in the field. He is the editor-in-chief of Autophagy. The November 2007 issue of Nature Reviews highlighted his article, "Autophagy: from phenomenology to molecular understanding in less than a decade." He is currently editing guidelines for the field, with 230 contributing authors, that will publish in Autophagy. Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal "house-cleaning" in the cell is called autophagy - literally self-eating. Breakthroughs in understanding the molecular basis of autophagy came after the cloning of ATG1 (autophagy-related gene 1) in yeast. (To date, 30 additional yeast genes have been identified.) These ATG genes in yeast were the stepping stones to the explosion of research into the molecular analysis of autophagy in higher eukaryotes. In the future, this research will help to design clinical approaches that can turn on autophagy and halt tumor growth. *Establishes the functional roles of specific cellular proteins in selective and nonselective autophagy in mammalian cells which aids researchers in determining why autophagy is shut down in neoplastia (growth of abnormal tissue mass) and turned on during bacterial invasion. *Includes methods to evaluate the role of autophagy in the drug-induced cell death of cancer cells in culture, which helps researchers design clinical approaches that can turn on autophagy and halt tumor growth. *Covers higher eukaryotes including lifespan in C. elegans to marine organisims and bridging into the clinical aspects, including autophagy in chronic myelogenous leukemia (CML is one of four types of leukaemia), lung cancer, prostate cancer, and cardiac cells.