Bibliothèque de L'IMIST/CNRST

Includes bibliographical references and index.

part 1. Key concepts for PCR-- Ch. 1. Optimization of PCR: conversations between Michael and David-- Ch. 2. The convergence of PCR, computers, and the human genome project: past, present, and future-- Ch. 3. Thermostable DNA polymerases: an update-- Ch. 4. Musings on microbial genomes-- Ch. 5. Statistical refinement of primer design parameters-- Ch. 6. Multiplex PCR: optimization guidelines-- Ch. 7. The use of immobilized mismatch binding protein for the optimization of PCR fidelity.

Ch. 8. A new generation of PCR instruments and nucleic acid concentration systems-- Ch. 9. Sequencing PCR products-- Ch. 10. Recent advances in high-temperature reverse transcription and PCR-- Ch. 11. Viral genotyping by a quantitative point mutation assay: application to HIV-1 drug resistance-- Ch. 12. In situ PCR-- part 2. Quantitative PCR-- Ch. 13. Standards for PCR assays-- Ch. 14. Rapid thermal cycling and PCR kinetics-- Ch. 15. Kinetics of competitive reverse transcriptase-PCR.

Ch. 16. Kinetic PCR analysis using a CCD camera and without using oligonucleotide probes-- Ch. 17. Quantification of telomerase activity using telomeric repeat amplification protocol-- part 3. Gene discovery-- Ch. 18. Differential display-- Ch. 19. Single-cell cDNA libraries-- Ch. 20. Whole cell assays-- Ch. 21. Screening differentially displayed PCR products by single-strand conformation polymorphism gels-- Ch. 22. Microsatellite protocols-- Ch. 23. Real-time quantitative PCR: uses in discovery research.

Ch. 24. Homology cloning: a molecular taxonomy of the archaea-- Ch. 25. Cloning mammalian homologs of drosophila genes-- Ch. 26. Cloning human homologs of yeast genes-- part 4. Genomics and expression profiling-- Ch. 27. Cellular transcriptome analysis using a kinetic PCR assay-- Ch. 28. Parallel analysis with biological chips-- Ch. 29. High-density cDNA grids for hybridization fingerprinting experiments-- Ch. 30. Comparative genomics hybridization-- Ch. 31. Genetic footprinting and functional maps of the yeast genome.

Ch. 32. Molecular analysis of microdissected tissue: laser capture microdissection-- Ch. 33. Amplified fragmant length polymorphism: studies on plant development-- Ch. 34. A florescent, multiplex solid-phase minisequencing method for genotyping cytochrome P450 genes-- Ch. 35. The Cleavase I enzyme for mutation and polymorphism scanning.

PCR is the powerful technique used in molecular biology. This book examines the developments in this field. It discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. It covers quantitative PCR techniques, including the use of standards.

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