Bibliothèque de L'IMIST/CNRST

Includes bibliographical references and index.

Introduction to enzyme science -- Active sites and their chemical properties -- Fundamentals of chemical kinetics -- Practical aspects of measuring initial rates and reaction parameters -- Initial-rate kinetics of one-substrate enzyme-catalyzed reactions -- Initial-rate kinetics of multi-substrate enzyme-catalyzed reactions -- Factors influencing enzyme activity -- Kinetic behavior of enzyme inhibitors -- Isotopic probes of biological catalysis -- Probing fast enzyme processes -- Regulatory behavior of enzymes -- Single-molecular enzyme kinetics -- Mechanoenzymes: catalysis, force generation and kinetics.

Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, this book develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes.