Bibliothèque de L'IMIST/CNRST

Currently Drosophila is a dominant experimental model in developmental biology and in gene regulation in eukaryotes. This volume summarizes some thirty years of experience in the handling of in vitro cultured Drosophila cells. Its main emphasis is on gene transfer methodology, cell responses to heat shock, hormonal regulation of genes, and on the expression and mobility of transposable elements. Key Features * Some thirty years of experience in handling in vitro cultured Drosophila cells * Cell cultures which provide material for a multiplicity of biochemical approaches * DNA-mediated gene transfer as an irreplaceable tool for analyzing basic mechanisms of regulation * Drosophila cell lines which qualify them for use in biotechnology.

(Chapter Headings) Composition of the Body Fluid of Drosophila and the Design of Culture Media for Drosophila Cells. Primary Cell Cultures of Drosophila Cells. Drosophila Continuous Cell Lines. Karyotype and Cell Cycle. Biology and Biochemistry of Cultured Cell Lines: Nucleic Acids. Biology and Biochemistry of Cultured Cell Lines: Proteins. Experimental Models of Gene Regulation: Heat-Shock Response of Drosophila Cells. Experimental Models of Gene Regulation: Cell Responses to Hormones. Gene Transfer Into Cultured Drosophila Cells. Transposons. Drosophila Viruses and Other Infections of Cultured Cells. Chapter References. Appendices. Bibliography. Subject Index.

"Bibliography": pages 597-690.

Includes bibliographical references and index.

Description based on print version record.