Laboratory methods in enzymology : RNA /
Collection : Methods in enzymology, 0076-6879 ; . volume 530 Mention d'édition :First edition. Détails physiques : 1 online resource (lviii, 413 pages) : illustrations (some color). ISBN :9780124200517 (electronic bk.); 0124200516 (electronic bk.).Includes bibliographical references and indexes.
Computational prediction of RNA secondary structure / Walter N. Moss -- Reverse-transcription PCR (RT-PCR) / Julia Bachman -- Northern blotting / Shan L. He and Rachel Green -- Explanatory chapter: nuclease protection assays / Elizabeth Eyler -- In vitro transcription from plasmid or PCR-amplified DNA / Julie L. Brunelle and Rachel Green -- Measurement of in vivo RNA synthesis rates / David S. McPheeters and Jo Ann Wise -- Method for measuring mRNA decay rate in Saccharomyces cerevisiae / Wenqian Hu and Jeff Coller -- Analysis of polysomes from bacteria / Daoming Qin and Kurt Fredrick -- Polysome profile analysis : yeast / Martin Pospísek and Leos Valásek -- Polysome analysis of mammalian cells / Shan L. He and Rachel Green -- Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae / Wenqian Hu and Jeff Coller -- Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates / Shimyn Slomovic and Gadi Schuster -- Circularized RT-PCR (cRT-PCR) : analysis of the 5' ends, 3' ends, and poly(A) tails of RNA / Shimyn Slomovic and Gadi Schuster -- RNA radiolabeling / Rishi Porecha and Daniel Herschlag -- Fluorescently labeling synthetic RNAs / Max Greenfield and Daniel Herschlag -- Analysis of RNA by analytical polyacrylamide gel electrophoresis / Alexey Petrov, Albet Tsa, and Joseph D. Puglisi -- RNA purification by preparative polyacrylamide gel electrophoresis / Alexey Petrov, Tinghe Wu, Elisabetta Viani Puglisi, and Joseph D. Puglisi -- Explanatory chapter : nucleic acid concentration determination / Sarah Kolitz and Jon Lorsch -- RNA purification : precipitation methods / Sarah E. Walker and Jon Lorsch -- Reverse transcriptase dideoxy sequencing of RNA / Sarah E. Walker and Jon Lorsch -- RNA structure experimental analysis : chemical modification / Zhili Xu and Gloria Culver -- Structural analysis of RNA backbone using in-line probing / Ali Nahvi and Rachel Green.
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