Bibliothèque de L'IMIST/CNRST

Includes bibliographical references and index.

Description based on print version record.

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect - full-text online of volumes 1 onwards. For more information about the Elsevier Book Series on ScienceDirect Program, please visit: http://www.info.sciencedirect.com/bookseries/ This volume is the first of two planned volumes on the topic of small GTPases and their role in disease.

1. Rab1b Silencing Using Small Interfering RNA for Analysis of Disease-Specific Function / Darren M. Hutt, William E. Balch -- 2. Rab8-Optineurin-Myosin VI: Analysis of Interactions and Functions in the Secretory Pathway / Margarita V. Chibalina ... [and others] -- 3. Characterization of Rab27a and JFC1 as Constituents of the Secretory Machinery of Prostate-Specific Antigen in Prostate Carcinoma Cells / Sergio D. Catz -- 4. In Vitro Assays to Characterize Inhibitors of the Activation of Small G Proteins by Their Guanine Nucleotide Exchange Factors / Jean-Christophe Zeeh ... [and others] -- 5. Analysis of Small GTPase Function in Trypanosomes / Mark C. Field, David Horn, Mark Carrington -- 6. Use of Dynasore, the Small Molecule Inhibitor of Dynamin, in the Regulation of Endocytosis / Tom Kirchhausen, Eric Macia, Henry E. Pelish -- 7. Identification and Verification of Sro7p as an Effector of the Sec4p Rab GTPase / Bianka L. Grosshans, Peter Novick -- 8. Characterization of Rab18, a Lipid Droplet-Associated Small GTPase / Sally Martin, Robert G. Parton -- 9. Analysis on the Emerging Role of Rab3 GTPase-Activating Protein in Warburg Micro and Martsolf Syndrome / Ayuko Sakane ... [and others]i

10. Identification and Characterization of JRAB/MICAL-L2, a Junctional Rab13-Binding Protein / Noriyuki Nishimura, Takuya Sasaki -- 11. A SMAP Gene Family Encoding ARF GTPase-Activating Proteins and Its Implication in Membrane Trafficking / Kenji Tanabe ... [and others] -- 12. In Vitro and In Vivo Analysis of Neurotrophin-3 Activation of Arf6 and Rac-1 / Pedro F. Esteban ... [and others] -- 13. Methods for Analysis of rab27a/munc13-4 in Secretory Lysosome Release in Hematopoietic Cells / Peter van der Sluijs ... [and others] -- 14. Analysis and Expression of Rab38 in Oculocutaneous Lung Disease / Kazuhiro Osanai, Dennis R. Voelker -- 15. Analysis of Rab1 Function in Cardiomyocyte Growth / Catalin M. Filipeanu, Fuguo Zhou, Guangyu Wu -- 16. Regulation of the Trafficking and Function of G Protein-Coupled Receptors by Rab1 GTPase in Cardiomyocytes / Guangyu Wu -- 17. Regulation of Endosome Dynamics by Rab5 and Huntingtin-HAP40 Effector Complex in Physiological versus Pathological Conditions / Arun Pal ... [et al].

19. Biochemical Characterization of Novel Germline BRAF and MEK Mutations in Cardio-Facio-Cutaneous Syndrome / Pablo Rodriguez-Viciana, Katherine A. Rauen -- 20. Biochemical and Biological Characterization of Tumor-Associated Mutations of p110 [alpha] / Adam Denley ... [and others] -- 21. Characterization of the Rheb-mTOR Signaling Pathway in Mammalian Cells: Constitutive Active Mutants of Rheb and mTOR / Tatsuhiro Sato, Akiko Umetsu, Fuyuhiko Tamanoi -- 22. Characterization of RalB-Sec5-TBK1 Function in Human Oncogenesis / Yuchen Chien, Michael A. White -- 23. High-Throughput Lung Cancer Cell Line Screening for Genotype-Correlated Sensitivity to an EGFR Kinase Inhibitor / Ultan McDermott, Sreenath V. Sharma, Jeffrey Settleman -- 24. In Vitro Signaling by MAPK and NF[kappa]B Pathways Inhibited by Yersinia YopJ / Sohini Mukherjee, Kim Orth -- 25. Biological and Biochemical Characterization of Anthrax Lethal Factor, a Proteolytic Inhibitor of MEK Signaling Pathways / Jennifer L. Bromberg-White, Nicholas S. Duesbery -- 26. Genetic Analyses of the Role of RCE1 in RAS Membrane Association and Transformation / Martin O. Bergo ... [and others] -- 27. Characterization of Kras-Mediated Pancreatic Tumorigenesis in Zebrafish / Jon M. Davison ... [and others] -- 28. Genetic Modeling of Ras-Induced Human Rhabdomyosarcoma / Corinne M. Linardic, Christopher M. Counter.