Bibliothèque de L'IMIST/CNRST

Cover; Title Page; Copyright Page; PREFACE; TABLE OF CONTENTS; INTRODUCTION; Chapter 1 Basic Physical Properties of DNA; I. DNA Structure; A. Single-Stranded DNA; B. The Double Helix; C. Alternative Forms of the Double Helix; D. Other Regular Structures; E. Hairpins and Cruciform Structures; II. Flexibility of the Double Helix; A. Macromolecular Conformation in Solution; B. Chains with Correlated Direction of Adjacent Links; C. Persistence Length of DNA; D. Excluded Volume Effect; E. Torsional Rigidity of the Double Helix; III. Conformational Transitions

A. General Properties of Conformational TransitionsB. Statistical-Mechanical Treatment of Conformational Transition; C. Helix-Coil Transitions; D. B-A Transition; E. B-Z Transition; Chapter 2 Circular DNA and Supercoiling; I. The Circular Form of DNA; A. The Discovery of Circular D N A; B. The Topological Aspect of Inter-Strand Linkage; C. Linking Number Difference and Superhelical Density: Topoisomers; II. Experimental Studies of Supercoiling; A. Determining the Linking Number Difference; B. The Dependence of Superhelical Density on Solution Conditions

C. Superhelicity of Circular DNA Isolated from CellsD. Obtaining DNA with a Preset Superhelical Density; III. Supercoiling Energy; IV. Topoisomerases; Chapter 3 Geometry and Topology of Circular DNA; I. Ribbon Theory; A. Writhe and Twist; B. Principal Properties of Writhe; II. Conformations of Supercoiled DNA; A. Torsional Rigidity of the Double Helix; B. Computer Simulation of Supercoiling; C. Experimental Studies of Supercoiled DNA Conformations; D. Thermodynamics of Supercoiling; III. Cyclization of DNA; IV. Knotted and Linked DNA; A. Types of Knots and Linkages

B. Theoretical Analysis of Knots and LinkagesC. Knots and Linkages in DNA; D. Topological Approach to the Study of the Mechanisms of Enzymatic Reactions; Chapter 4 Formation of Noncanonical Structures under the Impact of Negative Supercoiling; I. Experimental Methods of Analysis of Noncanonical Structures in Circular DNA; A. Methods of Localization of Structural Transitions; B. The Method of Two-Dimensional Gel Electrophoresis; II. Thermodynamic Analysis of the Formation of Noncanonical Structures; III. Cruciform Structures; IV. The Left-Handed Z-Form; V. Melting of Closed Circular DNA

VI. The H-FormVII. The Influence of Supercoiling on the B-A Transition; VIII. Statistical-Mechanical Analysis; IX. Mutual Influence and Competition of Different Structural Transitions; X. Kinetics of the Formation of Noncanonical Structures in Circular DNA; Chapter 5 The Use of Circular DNA in Studies of the Double Helix and Its General Properties; I. Finding the Helical Repeat of the Double Helix; II. Finding the Double Helix Unwinding Angle upon Ligand Binding; Chapter 6 DNA Supercoiling Inside the Cell; I. Superhelical Stress; II. The Existence of Noncanonical Structures within a Cell