Bibliothèque de L'IMIST/CNRST

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Analytical ultracentrifugation : techniques and methods / par SCOTT, David Publication : [S.l.] : Royal Society of Chemistry, 2006 . 580 p. ; , Analytical Ultracentrifugation has become an increasingly important technique for monitoring the size and shape of biological macromolecules. "Analytical Ultracentrifugation: Techniques and Methods" contains contributions from experts in the field, bringing together the multitude of developments that have taken place in instrumentation and analysis over the past decade into a single volume. This book covers the latest methods in analysis along with an extensive introduction for the novice user. Analysis methods in both sedimentation velocity and sedimentation equilibrium are discussed at length. Protein, Protein/DNA, membrane proteins and polymer systems are also explored, along with software developments and non-ideality. 24 cm. Date : 2006 Disponibilité : Exemplaires disponibles: La bibliothèque des Sciences Exactes et Naturelles (1),

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Mammalian alkaline phosphatases : from biology to applications in medicine and biotechnology / par Millan,, Jos? Luis. Publication : [S.l.] : Wiley-VCH, 2006 . 337 p. ; , A review and discussion of new knowledge on the structure and function of mammalian alkaline phosphatases (APs) gained over the last 25 years. It covers: The structure, regulation and expression of the AP genes The three-dimensional structure of APs and mutagenesis work that further defined the structural/functional domains of the isozymes The phenotypic abnormalities of the different AP knockout mice Our current understanding of the in vivo role of the AP isozymes. The book also describes the possible use of APs as therapeutic agents and therapeutic targets and the many uses of these enzymes in clinical medicine and in biotechnology. 25 cm. Date : 2006 Disponibilité : Exemplaires disponibles: La bibliothèque des Sciences Exactes et Naturelles (1),

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Molecular diagnostics : current technology and applications / par Rao,, Juluri R. Publication : [S.l.] : Taylor & Francis, 2006 . 400 p. ; , Molecular techniques are being harnessed for the detection of organisms in a wide range of circumstances. Global trade has introduced new pests of crops and plants; simultaneously, there is universal concern from disease outbreaks such as BSE, Foot & Mouth and the dreaded bird-flu virus. Molecular diagnostic applications for the detection of foodborne bacterial pathogens and genetically modified organisms in food are also becoming increasingly important. This book provides a critical assessment of the various molecular methods in medical and veterinary science, forensics, biodefense, plant science, food safety and quarantine sciences. Aimed at a wide range of end-users encompassing postgraduates, postdoctoral research scientists, established researchers and specialist scientists, the authors describe and evaluate molecular techniques and applications in this increasingly important area. Topics include the molecular methods for the direct detection of pathogens such as E. coli O157:H7, MRSA and other important bacterial infections, medical viruses, medical parasites, dental plaque bacteria and haematological malignancies. Also covered is the molecular recognition and detection of catastrophic bio-threat agents as a consequence of global terrorism. 24 cm. Date : 2006 Disponibilité : Exemplaires disponibles: La bibliothèque des Sciences Médicales et Pharmaceutiques (1),

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Peroxiredoxin systems : structures and functions / par Flohé,, Leopold. Publication : [S.l.] : Springer, 2007 . 410 p. ; , The peroxiredoxins represent an important emerging family of sulfhydryl-linked antioxidant proteins, apparently ubiquitously present in all known organisms. The antioxidant function of the peroxiredoxins complements that of other enzymic and non-enzymic systems within the cell. In addition, there is increasing evidence that the peroxiredoxins play a role in cell signalling, by controlling and/or sensing hydrogen peroxide and peroxynitrite levels. In this, the first book containing a broad survey on the peroxiredoxins, the editors succeeded to involve almost all groups that contributed significant insights into the emerging field. The diverse biological roles of the new protein family are discussed in the context of other antioxidant systems like those based on heme or selenium catalysis. Finally, related future perspectives, which range from antimicrobial therapy up to the understanding of human development and carcinogenesis, are highlighted. Specifically, the book will be of outstanding interest to researchers who want to understand how micro-organisms, plants and animals cope with oxidative stress and how such stress is recognized and linked to adaptive responses via redox regulation of cellular processes. 24 cm. Date : 2007 Disponibilité : Exemplaires disponibles: La bibliothèque des Sciences Médicales et Pharmaceutiques (1),

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Plant biochemistry par Heldt, Hans-Walter. Publication : [S.l.] Academic Press 2005 . 630 p. , This fully revised translation of the world-renowned German edition covers the complete and modern knowledge of plant biochemistry. The book presents the topic in a concise and simplified manner so that students can digest the message and gain a basic knowledge of the entire field of plant biochemistry, from photosynthesis (the synthesis of natural plant products) to all kinds of genetic engineering with its many commercial applications. Topics include cell structure and function, lipid and polysaccharide metabolism, nitrogen fixation, phloem transport, synthesis and function of isoprenoids, phenylpropanoids and other secondary metabolites, plant growth regulation and development. Heldt provides a comprehensive description of photosynthesis, primary and secondary metabolism, the function of phytohormones and molecular engineering. The text covers research findings until the end of 2003 and identifies areas of future research. There are extensive references to the most recent scientific literature. * Illustrated with two-color diagrams and well designed metabolic schemes * Plant metabolism presented in the context of the structure and function of the plant * Many examples of commercial applications for the protection and quality improvement of harvest crops, plant breeding, and the production of raw materials for industrial use * Coverage of the latest research findings. 27 cm. Date : 2005 Disponibilité : Exemplaires disponibles: La bibliothèque des Sciences Exactes et Naturelles (1),

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Serial analysis of gene expression.   Publication : [S.l.] : Humana Press, 2007 . 208 p. ; , Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures the expression of both known and unknown genes. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world. Part 1 is dedicated to experimental procedures of SAGE and related methods including aRNA LongSAGE, SuperSAGE, DeepSAGE, and GMAT. Part 2 provides methods for extraction and filtration of tags, analysis of ditag populations, and completing statistically correct comparisons of gene expression profiles. Comparative transcriptomics enables scientists to understand the underlying genetics of biological changes such as development, disease, crop yield, and resistance. SAGE analysis is also used to obtain unknown tags, which can be used as gene-specific primers in Rapid Amplification of cDNA Ends (RACE) reactions to generate full-length transcripts for cloning and sequencing. This book will be an indispensable tool for any lab engaged in genetic profiling and comparative transcriptomics, and will help many laboratories to successfully implement tag-based sequencing methods and procedures and obtain comprehensive, useful, and interpretative data. 23 cm. Date : 2007 Disponibilité : Exemplaires disponibles: La bibliothèque des Sciences Exactes et Naturelles (1),

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