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Fluorescence spectroscopy / edited by Ludwig Brand, Michael L. Johnson. - 1 online resource (xlii, 358 pages, [8] pages of plates) : illustrations (some color) - Methods in enzymology, v. 450 0076-6879 ; . - Methods in enzymology ; v. 450. .

Includes bibliographical references and indexes.

A method in enzymology for measuring hydrolytic activities in live cell environments / Beverly Z. Packard and Akira Komoriya -- Heterogeneity of fluorescence determined by the method of area-normalized time-resolved emission spectroscopy / N. Periasamy -- Multiparametric probing of microenvironment with solvatochromic fluorescent dyes / Andrey S. Klymchenko and Alexander P. Demchenko -- Site-selective red-edge effects / Alexander P. Demchenko -- Fluorescence approaches to quantifying biomolecular interactions / Catherine A. Royer and Suzanne F. Scarlata -- Forster resonance energy transfer measurements of transmembrane helix dimerization energetics / Mikhail Merzlyakov and Kalina Hristova -- Application of single-molecule spectroscopy in studying enzyme kinetics and mechanism / Jue Shi ... [and others] -- Ultrafast fluorescence spectroscopy via upconversion : applications to biophysics / Jianhua Xu and Jay R. Knutson -- Use of fluorescence resonance energy transfer (FRET) in studying protein-induced DNA bending / Anatoly I. Dragan and Peter L. Privalov --Fluorescent pteridine probes for nucleic acid analysis / Mary E. Hawkins -- Single-molecule fluorescence methods for the analysis of RNA folding and ribonucleoprotein assembly / Goran Pljevaljčić and David P. Millar -- Using fluorophore-labeled oligonucleotides to measure affinities of protein-DNA interactions / Brian J. Anderson ... [and others] -- Identifying small pulsatile signals within noisy data : a fluorescence application / Michael L. Johnson ... [and others] -- Determination of zinc using carbonic anhydrase-based fluorescence biosensors / Rebecca Bozym ... [and others] -- Instrumentation for fluorescence-based fiber optic biosensors / Richard B. Thompson ... [and others].

Fluorescence spectroscopy is a type of electromagnetic spectroscopy, using a beam of light, which analyzes fluorescence from a sample. Given its extremely high sensitivity and selectivity, it is an important investigational tool in many areas including material sciences, analytical sciences, and across a broad range of chemical, biochemical and medical research. It has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems. The pharmaceutical industry uses it heavily and it has become a dominating technique in biochemistry and molecular genetics. * Keeps MIE buyers and online subscribers up-to-date with the latest research with this highly used technique. * Provides tried and tested techniques which eliminate searching through many different sources.

9780123745866 (electronic bk.) 0123745861 (electronic bk.)


Fluorescence spectroscopy.
Spectrometry, Fluorescence--methods.
Fluorescence spectroscopy.


Electronic books.

QP601 / .M49450 2008eb

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