Laboratory methods in enzymology : DNA /
Collection : Methods in enzymology, 0076-6879 ; . volume 529 Mention d'édition :First edition. Détails physiques : 1 online resource (lvi, 348 pages) : illustrations. ISBN :9780124199545 (electronic bk.); 0124199542 (electronic bk.).
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DNA.
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Biotechnology
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Methodology.
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Molecular biology
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Methodology.
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Sequence Analysis, DNA
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methods.
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Biotechnology
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methods.
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Biotechnology
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istrumentation.
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Molecular biology
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methods.
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Molecular biology
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istrumentation.
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SCIENCE / Life Sciences / Biochemistry
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Electronic books.
Includes bibliographical references and indexes.
Explanatory chapter: PCR primer design / Rubén Álvarez-Fernández -- Explanatory chapter: How plasmid preparation kits work / Laura Koontz -- Explanatory chapter: Introducing exogenous DNA into cells / Laura Koontz -- Agarose gel electrophoresis / Laura Koontz -- Analysis of DNA by southern blotting / Gary Glenn and Lefkothea-Vasiliki Andreou -- Purification of DNA oligos by denaturing polyacrylamide gel electrophoresis (PAGE) / Sara Lopez-Gomollon and Francisco Esteban Nicolas -- Molecular cloning / Juliane C. Lessard -- Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway® recombination cloning technology and Flp-In T-REx® lines / Jessica Spitzer, Markus Landthaler, and Thomas Tuschl -- Restrictionless cloning / Mikkel A. Algire -- Isolation of plasmid DNA from bacteria / Lefkothea-Vasiliki Andreou -- Preparation of genomic DNA from bacteria / Lefkothea-Vasiliki Andreou -- Preparation of genomic DNA from Saccharomyces cerevisiae / Jessica S. Dymond -- Isolation of genomic DNA from mammalian cells / Cheryl M. Koh -- Sanger dideoxy sequencing of DNA / Sarah E. Walker and Jon Lorsch -- Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform / Srinivasan Yegnasubramanian -- Explanatory chapter: Next generation sequencing / Srinivasan Yegnasubramanian -- Generating mammalian stable cell lines by electroporation / Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- Transient mammalian cell transfection with polyethylenimine (PEI) / Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- Site-directed mutagenesis / Julia Bachman -- PCR-based random mutagenesis / Jessica S. Dymond -- Megaprimer method for mutagenesis of DNA / Craig W. Vander Kooi -- Explanatory chapter: Troubleshooting PCR / Kirstie Canene-Adams -- Explanatory chapter: Quantitative PCR / Jessica S. Dymond -- General PCR / Kirstie Canene-Adams -- Colony PCR / Megan Bergkessel and Christine Guthrie -- Chemical transformation of yeast / Megan Bergkessel and Christine Guthrie -- Transformation of E. coli via electroporation / Juliane C. Lessard -- Transformation of chemically competent E. coli / Rachel Green and Elizabeth J. Rogers.
Description based on print version record.
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