Bibliothèque de L'IMIST/CNRST

Front Cover; Fluorescence-based Biosensors: From Concepts to Applications; Copyright; Contents; Contributors; Preface; Introduction; References; Chapter 1: The Chemistry of Small-Molecule Fluorogenic Probes; 1. Introduction; 2. Fluorescence and Fluorophores; 3. Modes of Fluorescence Modulation; 4. Coumarins; 4.1. Overview; 4.2. Fluorogenic enzyme substrates; 4.3. Photoactivatable probes; 4.4. Indicators; 5. BODIPY Dyes; 5.1. Overview; 5.2. Enzyme substrates; 5.3. Indicators; 6. Fluoresceins; 6.1. Overview; 6.2. Enzyme substrates; 6.3. Photoactivatable probes; 6.4. Indicators; 7. Rhodamines.

7.1. Overview7.2. Enzyme substrates; 7.3. Photoactivatable probes; 7.4. Indicators; 8. Phenoxazine Dyes; 8.1. Overview; 8.2. Enzyme substrates; 8.3. Photoactivatable probes; 8.4. Indicators; 9. Acridinones; 9.1. Overview; 9.2. Fluorogenic enzyme substrates and photoactivatable probes; 10. Cyanines; 10.1. Overview; 10.2. Enzyme substrates; 10.3. Photoactivatable probes; 10.4. Indicators; 11. Other Fluorogenic Scaffolds; 11.1. Overview; 11.2. Fluorogenic enzyme substrates; 11.3. Photoactivatable probes; 11.4. Indicators; 12. Conclusions and Future Directions; References.

Chapter 2: Fluorescent Environment-Sensitive Dyes as Reporters of Biomolecular Interactions1. Introduction; 2. Single-Band Solvatochromic Dyes: Design and Applications; 2.1. Naphthalene sulfonic acid derivatives; 2.2. Prodan; 2.3. Improved Prodan analogues; 2.4. 4-Aminophthalimide analogues; 2.5. Fluoroprobe; 2.6. Dapoxyl® derivatives; 2.7. NBD; 2.8. Phenoxazine derivatives; 2.9. New advanced solvatochromic dyes; 3. Two-Band Solvatochromic Dyes Based on ESIPT; 4. Applications of Two-Color Dyes for Monitoring Biomolecular Interactions; 4.1. Monitoring conformational changes of proteins.

4.2. Peptide-nucleic acid interactions4.3. Protein-protein interactions; 4.4. Peptide-membrane interactions; 5. Conclusions; References; Chapter 3: Rational Design of Fluorophores for In Vivo Applications; 1. Introduction; 2. Cyanine and Related Fluorophores; 2.1. General characterization; 2.2. Bioconjugation; 2.3. Improvement of water solubility and chemical stability; 2.3.1. Chemical modifications; 2.3.2. Encapsulation; 3. Squaraines; 4. Boron Dipyrromethene and Related Fluorophores; 4.1. General characterization; 4.2. Energy-transfer dyads for increasing the Stokes shift of BDPs.

4.3. Related fluorophores5. Porphyrins, Phthalocyanines, and Related Macrocycles; 5.1. General characterization; 5.2. Benzoporphyrins; 5.3. Strongly conjugated porphyrin arrays; 5.4. Chlorins and bacteriochlorins; 5.5. Hydroporphyrin arrays for increased Stokes shift and multicolor in vivo imaging; 5.6. Water solubility and aggregation; 5.7. Photocytotoxicity of tetrapyrrolic macrocycle; 5.8. Phthalocyanines; 6. Special Types of Fluorophores; 6.1. Fluorophores for multicolor imaging; 6.2. Self-illuminating fluorophores; 7. Conclusion; Acknowledgment; References.

Chapter 4: Fluorescence Technologies for Monitoring Interactions Between Biological Molecules In Vitro.

One of the major challenges of modern biology and medicine consists in finding means to visualize biomolecules in their natural environment with the greatest level of accuracy, so as to gain insight into their properties and behaviour in a physiological and pathological setting. This has been achieved thanks to the design of novel imaging agents, in particular to fluorescent biosensors. Fluorescence Biosensors comprise a large set of tools which are useful for fundamental purposes as well as for applications in biomedicine, drug discovery and biotechnology. These tools have been de.

Description based on print version record.