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Serial analysis of gene expression.   Publication : [S.l.] : Humana Press, 2007 . 208 p. ; , Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures the expression of both known and unknown genes. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world. Part 1 is dedicated to experimental procedures of SAGE and related methods including aRNA LongSAGE, SuperSAGE, DeepSAGE, and GMAT. Part 2 provides methods for extraction and filtration of tags, analysis of ditag populations, and completing statistically correct comparisons of gene expression profiles. Comparative transcriptomics enables scientists to understand the underlying genetics of biological changes such as development, disease, crop yield, and resistance. SAGE analysis is also used to obtain unknown tags, which can be used as gene-specific primers in Rapid Amplification of cDNA Ends (RACE) reactions to generate full-length transcripts for cloning and sequencing. This book will be an indispensable tool for any lab engaged in genetic profiling and comparative transcriptomics, and will help many laboratories to successfully implement tag-based sequencing methods and procedures and obtain comprehensive, useful, and interpretative data. 23 cm. Date : 2007 Disponibilité : Exemplaires disponibles: La bibliothèque des Sciences Exactes et Naturelles (1),

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Textbook of respiratory cell and molecular biology / par Wardlaw,, A. J. Publication : [S.l.] : Informa Healthcare, 2002 . 500 p. ; , The dramatic advances made in molecular science and genetics during the last two decades have started to transform our understanding of the scientific basis of human disease. We are now at the threshold of understanding, at the level of an individual protein or gene, what has gone wrong and how we might put it right. This is as true in respiratory disease as in other specialities. The genetic basis for cystic fibrosis is now clear and gene replacement therapy is a real possibility; analysis of the fine structure of *a1-antitrypsin has revealed how mutations cause disease; and the secrets of how respiratory syncytial virus causes bronchiolitis in children are being unlocked. However, as the literature expands at an exponential rate, clinicians who may have last studied immunology or genetics at medical school, or scientists new to respiratory research, can find it increasingly difficuly to keep up-to-date. This book is aimed primarily at those with a special interest in respiratory disease, either as physicians or scientists, who want a detailed but clear explanation of our current understanding of the pathogenesis of common respiratory disease. While the book makes no apologies for exploring in detail the scientific complexities that underpin respiratory disease, it will have failed if it is not also an enjoyable and stimulating read. Although much remains to be known, this book provides both a reference and a benchmark against which future progress can be measured. 25 cm. Date : 2002 Disponibilité : Exemplaires disponibles: La bibliothèque des Sciences Exactes et Naturelles (1),

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The Evolution of plants / par Willis,, K. J. Publication : [S.l.] : Oxford University Press, USA, 2002 . 392 p. ; , This is a broad but provocative examination of the evolution of plants from the earliest forms of life to the development of our present flora. Taking a fresh, modern approach to a subject often treated very stuffily, the book incorporates many recent studies on the morphological evolution of plants, enlivens the subject with current research on ancient DNA and other biomolecular markers, and places plant evolution in the context of climate change and mass extinction. It is written to be accessible to undergraduates, so, for example, geological time is discussed in terms of 'millions of years ago' as well as by the names of the ages, and English equivalents of plant names are prefered, e.g. seed plants (instead of gymnosperms), flowering plants (instead of angiosperms). 25 cm. Date : 2002 Disponibilité : Exemplaires disponibles: La bibliothèque des Sciences Exactes et Naturelles (1),

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The World of the cell / par Kleinsmith, Lewis J. Publication : [S.l.] : Benjamin-Cummings Publishing Company, Subs of Addison Wesley Longman, Inc, 2005 . 944 p. ; , Relié. 28 cm. Date : 2005 Disponibilité : Exemplaires disponibles: La bibliothèque des Sciences Exactes et Naturelles (1),

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